Thermo Fisher, C606010, One Shot™ BL21(DE3)pLysS Chemically Competent E. coli

11 x 50 μL One Shot BL21(DE3)pLysS Chemically CompetentE. colicells are ideal for use with bacteriophage T7 promoter-based expression systems such as pRSET and pET. The pLysS plasmid carried by the BL21(DE3) pLysS strain produces T7 lysozyme, an inhibitor of T7 RNA polymerase, to reduce basal level expression of the gene of interest. pLysS confers resistance to chloramphenicol (CamR) and contains the p15A origin. This origin allows pLysS to be compatible with plasmids containing the ColE1 or pMB1 origin (i.
e. pUC- or pBR322- derived plasmids). Recombinant proteins that are nontoxic toE. coliare generally expressed at higher levels in BL21(DE3) cells compared to the BL21(DE3) pLysS strain with tighter expression control. BL21(DE3)pLysS is recommended for use when expressing toxic genes.
The BL21 (DE3)E. colistrain is one of the most popular host strains to produce recombinant proteins. This strain had been engineered to carry the λDE3 lysogen that contains T7 RNA polymerase gene under control of thelacUV5 promoter. IPTG is required to induce expression of the T7 RNA polymerase cascade system that in turn transcribe T7 promoter regulated target gene.
BL21(DE3) is a derivative from theE. coliB strain that does not contain the ion protease and is also deficient in the outer membrane protease OmpT. The lack of two key proteases reduces degradation of heterologous proteins expressed in the strains. Additionally, several other strain features make them especially suited for protein production, namely fast growth in minimal medium, a lower acetate production when grown in high glucose media, and an ability to reach high cell density. One Shot BL21(DE3)pLysS Chemically CompetentE. colicells can achieve transformation efficiency of >1 x 108cfu/μg of control DNA.
One Shot BL21(DE3)pLysS Chemically CompetentE. colicells offer:
• Transformation efficiency of >1 x 108cfu/μg control plasmid DNA
• Ion and OmpT protease deficiency that reduces degradation of recombinant protein
• IPTG-induciblelacUV5 promoter that controls expression of the T7 RNA polymerase
• pLysS (CamR) plasmid for reduced basal expression in uninduced cells
•hsdS mutation that allows efficient transformation of unmethylated DNA
• Fast growth in minimal medium and ability to reach high cell densityEasy-to-use One Shot formatBL21(DE3)pLysS Chemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and to prevent contamination.
GenotypeF–ompThsdSB (rB–, mB–)gal dcm(DE3) pLysS(CamR)Find the strain and format that you needWe offer otherE. colistrains for protein expression.
For expression of toxic proteins, considerBL21 AI One Shot Chemically CompetentE. coli.
BL21 Star (DE3) and other strains are available inMultiShot formats for high throughput applications.
Explorebacterial growthmedia formats.
We offer a variety ofsystems for the expression of recombinant proteinsinE. coli.
TheChampion pET expression systemprovides the highest level of protein production available in any expression system.
Antibiotic Resistance Bacterial: Yes (Chloramphenicol)
Blue/White Screening: No
Cloning Methylated DNA: No
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: No
Improves Protein Stability: Yes (lon,ompT)
Improves RNA Stability: Yes (pLysS)
Preparing Unmethylated DNA: Yes (dcm)
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 11 x 50 μL/tube
Reduces Recombination: No
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Toxic Proteins: No
Transformation Efficiency Level: Medium Efficiency (1 x 108to 1 x 109cfu/μg)
Format: Tube
Promoter: T7
Species: E. coli (B)
Unit Size: Each