Thermo Fisher, C66303, MC1061/P3 Chemically Competent E. coli

MC1061/P3 Chemically Competent E. coli MC1061/P3 Chemically CompetentE. coliare used for highly efficient transformations that require the P3 episome for selection and maintenance of vectors encoding the tyrosine tRNA suppressor (synthetic supF gene, such as pCDM8, pcDNA1.
1, or any othersupF-containing vector).
MC1061E. colicells were used by Hanahan and coworkers to engineer the DH10B strain. Among the replaced genes by adding mutations wererecA1 (inhibiting the homologous recombination system),endA1, andlacZΔM15. Thus, this strain with its intact homologous recombination system is used in applications where recombination is needed, such as in genetically engineeringE. coliby recombination or plasmid-phage recombination.
MC1061/P3 Chemically CompetentE. colioffer:
• Transformation efficiencies of >1 x 108cfu/μg
• P3 plasmid for selection ofsupF-containing plasmids
•hsdR for efficient transformation of unmethylated DNA from PCR amplificationsP3 plasmidThe P3 plasmid is a low-copy-number, 60 kb plasmid that carries kanamycin, tetracycline, and ampicillin resistance markers. The kanamycin gene is fully active and used to select for cells carrying P3. The tetracycline and ampicillin genes carry amber mutations, which render the genes inactive during normal growth and replication of the bacteria. Upon transformation of a vector carrying the suppressor F gene, the amber mutations in the tetracycline and ampicillin genes on the P3 plasmid are suppressed, and theE. coliare resistant to these antibiotics.
Note:The spontaneous reversion rate for the amber mutation in the ampicillin marker is 5%, while the reversion rate for the amber mutation in the tetracycline marker is 1%.
To minimizing the reversion rates, use tetracycline and ampicillin together for selection ofE. coli(P3) cells transformed with the SupF plasmid.
GenotypeF–hsdR(rK–, mK+)araD139 Δ(araABC-leu)7679galUgalK ΔlacX74rpsL(StrR)thi mcrB / P3: KanRAmpR(am) TetR(am)Find the strain and format that fits your needsWe offer DH strains inchemically competentandelectrocompetent cellformats to meet your specific needs.
TheTOP10/P3 straincould be used where recombination is not needed.
Strains are available in severalMultiShot formatsfor high throughput applications.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: Yes (Streptomycin, Kanamycin, Ampicillin, Tetracycline)
Blue/White Screening: No
Cloning Methylated DNA: Yes (hsd)
Cloning Unstable DNA: No
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: No
Preparing Unmethylated DNA: No
Product Type: Chemically Competent Cells
Quantity: 5 x 300 μL
Reduces Recombination: No
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: Medium Efficiency (1 x 108to 1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each