Thermo Fisher, C66455, TOP10 Electrocomp™ Kit

5 x 100 μL TOP10 ElectrocompE. colicells are provided at a transformation efficiency of 1 x 1010cfu/μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. This strain is used in wide range of applications and allows stable replication of high-copy number plasmids and maintenance of large plasmids.
The TOP10 genotype is similar to the DH10B strain and features:
•hsdR for efficient transformation of unmethylated DNA from PCR amplifications
•mcrA for efficient transformation of methylated DNA from genomic preparations
•lacZΔM15 for blue/white color screening of recombinant clones
•endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
•recA1 for reduced occurrence of non-specific recombination in cloned DNANotesPlease see the product manual for reaction scale recommendations. A high-voltage electroporation apparatus is required.
GenotypeF-mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1araD139 Δ(ara-leu)7697galUgalKrpsLendA1nupGFind the strain and format that fits your needsTOP10 is available inchemically competentandelectrocompetent cellformats.
ChooseMultiShot competent cellsfor high-throughput cloning applications.
Email usfor more information about custom formats.
Antibiotic Resistance Bacterial: Yes (Streptomycin)
Blue/White Screening: Yes (lacΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: No
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: May be used for plasmids >20 kb
Preparing Unmethylated DNA: No
Product Line: Electrocomp™
Product Type: Electrocompetent Cells
Quantity: 5 x 100 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency Level: High Efficiency (>1 x 1010cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each