Thermo Fisher, C66511, TOP10F' Electrocomp™ Kit

TOP10F' Electrocomp™ Kit TOP10F´ Electrocomp KitE. colicells are identical to TOP10 cells, with the addition of an F´ episome. The F´ episome carries the tetracycline resistance gene and allows isolation of single-stranded DNA (ssDNA) from vectors that have an f1 origin of replication. In addition, the F´ episome carries thelacIq repressor for inducible expression fromtrc,tac, andlacpromoters using IPTG.
TOP10F´ competent cells are transformed via electroporation, thus achieving higher transformation efficiencies than chemically competent cells and may be used in more challenging applications, including cloning a low amount of DNA or cDNA and gDNA library construction. These cells allow stable replication of high copy number plasmids and are provided at a transformation efficiency of >1 x 1010cfu/ μg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation.
As a derivative of TOP10, TOP10F´ cells carry mutations in the methylation-dependent restriction system (mcrA,mcrBC, andmrr) allowing one to clone both prokaryotic and eukaryotic genomic DNA, as well as efficient plasmid rescue from eukaryotic genomes. Similar to other DH strains, the TOP10 strain also hasrecA1 andendA1 mutations to increase insert stability and improve the quality of plasmid DNA prepared from minipreps.
TOP10F´ Electrocomp KitE. colicells offer:
• Transformation efficiencies of >1 x 1010cfu/μg
• Stable F´ episome that allows isolation of ssDNA and carrieslacIq repressor for inducible expression fromtrc,tac, andlacpromoters
•hsdR for efficient transformation of unmethylated DNA from PCR amplifications
•mcrA for efficient transformation of methylated DNA from genomic preparations
•lacZΔM15 for blue/white color screening of recombinant clones on plates containing either X-Gal or Bluo-Gal
•endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
•recA1 for reduced occurrence of nonspecific recombination in cloned DNANote:TOP10F´ cells require IPTG induction for blue-white color screening. A high-voltage electroporation apparatus is also required.
GenotypeF´ [lacIq,Tn10(TetR)]mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74recA1araD139 Δ(ara-leu)7697galUgalKrpsL(StrR)endA1nupGFind the strains and formats that fit your needsWe offer other DH strains inchemically competentandelectrocompetent cell.
Electroporation cuvettes with0.
1 cm,0.
2 cm, and0.
4 cmgaps are available.
The TOP10 strain is available in severalMultiShot formatsfor high throughput applications.
We offer other strains for ssDNA production, such asDH12S.
Explorebacterial growthmedia formats.
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Improves Plasmid Quality: Yes (endA1)
Plasmid: May be used for plasmids >20 kb
Preparing Unmethylated DNA: No
Product Line: Electrocomp™
Product Type: Electrocompetent Cells
Quantity: 10 x 100 μL
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
Transformation Efficiency Level: High Efficiency (>1010cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each