Thermo Fisher, C717103, One Shot™ INV110 Chemically Competent E. coli

One Shot™ INV110 Chemically Competent E. coli One Shot INV110 Chemically CompetentE. colicells are derived from the JM110 strain and are specifically designed for the growth and purification of plasmid DNA that will be digested withdamordcmmethylation-sensitive restriction enzymes. Thedamanddcmdeficiency allows for the production of plasmid DNA that is unmethylated at GmATC and CmC(A/T)GG sites. INV110 Chemically Competent cells can achieve a transformation efficiency of >1 x 106cfu/μg of control DNA.
The INV110 strain is a significant improvement over the JM110 strain. The INV110 strain has been engineered to carry anendA1 mutation, which eliminates the non-specific Endonuclease I, for improved plasmid DNA preparation. Mutation in the methylation-dependent restriction system Δ(mcrC-mrr) allows transformation of genomic DNA. This strain also has thelacZΔM15 genotype, providing for the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. The INV110 strain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids) and allows isolation of single-stranded DNA (ssDNA). In addition, the F´ episome carries thelacIq repressor for inducible expression fromtrc,tac, andlacpromoters using IPTG.
One Shot INV110 Chemically CompetentE. colioffer:
• Transformation efficiencies of >1 x 106cfu/μg
•damanddcmdeficiency to allow restriction digestion with dam and dcm-sensitive restriction enzymes
• Stable F´ episome that allows isolation of ssDNA and carrieslacIq repressor for inducible expression fromtrc,tac, andlacpromoters
• Δ(mcrC-mrr) mutation that reduces cleavage of foreign methylated DNA and improves transformation of genomic DNA
•lacZΔM15 for blue-white color screening of recombinant clones
•endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
• Tn10 to permit selection of the host strain using tetracyclineNote:damdeletion results in higher mutation rate, higher recombination frequency, and lower viability on plates. Avoid picking colonies that are noticeably larger than the rest of the population (potential revertants). Pick colonies and isolate the DNA within one day of transformation.
Convenient and efficient formatOne Shot INV110 Chemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation, reducing both freeze-thaw cycles and costs associated with unused cells.
GenotypeF´ [traD36proABlacIqlacZΔM15]rpsL (StrR)thr leu endAthi-1lacYgalKgalTara tonAtsx dam dcm supE44 Δ(lac-proAB) Δ(mcrC-mrr)102::Tn10(TetR)Find the strain and format that fits your needsWe offer other strains inchemically competentandelectrocompetent cellformats to meet your specific needs.
Strains inMultiShot formatsfor high throughput applications are available.
Alternative strains for ssDNA production are available, such asTOP10F´or electrocompetentDH12S.
Explorebacterial growthmedia formats.
Explorerestriction enzyme detailsto find methylation sensitivity.
Antibiotic Resistance Bacterial: Yes (Streptamycin, Tetracycline)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcr, mrr)
Cloning Unstable DNA: No
Contains F' Episome: Yes
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 21 x 50 μL/tube
Reduces Recombination: No
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency Level: Subcloning Efficiency (1 x 106to 1 x 107cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each