Thermo Fisher, C854003, One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli

One Shot™ OmniMAX™ 2 T1R Chemically Competent E. coli The One Shot OmniMAX 2 T1RE. colistrain is an improved chemically competent cell line that can achieve extremely high transformation efficiency of >5 x 109transformants/μg pUC19 DNA. It offers the highest transformation efficiency of any chemically competent cells in a One Shot format. OmniMAX 2 T1Ris a versatile strain, perfect to use in all cloning applications, including Gateway technology and TOPO PCR cloning.
OmniMAX 2 T1RChemically CompetentE. colicells carry thetonA (also known asfhuA) mutation, which confers resistance to T1 and T5 phage infection. ThetonA genotype offers added security to valuable clones and libraries. This strain lacks theE. coliK12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)), allowing efficient transformation of highly methylated DNA. The OmniMAX 2 T1Rstrain has thelacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries therecA1 mutation, which helps reduce the rate of recombination while propagating plasmid DNA, and theendA1 mutation that increases plasmid DNA yield and quantity. The OmniMAX 2 T1Rstrain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids). In addition, the F´ episome carries thelacIq repressor for inducible expression fromtrc,tac, andlacpromoters using IPTG. Importantly, the ccdA gene present on the F´ episome is deleted, allowing negative selection of Gateway vectors as the strain is sensitive to theccdB gene product.
One Shot OmniMAX 2 T1RChemically CompetentE. colioffer:
• Highest transformation efficiencies of >5 x 109cfu/μg
•tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Δ(ccdAB) for sensitivity to the toxic effects of theccdB gene product, allowing negative selection of vectors containing theccdB gene (Gateway cloning system)
• Stable F´ episome that carrieslacIq repressor for inducible expression fromtrc,tac, andlacpromoters
• Construction of more representative genomic libraries due to the elimination ofmcrA,mcrBC,mrr, andhsdRMS
•lacZΔM15 for blue-white color screening of recombinant clones
•endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
•recA1 for reduced occurrence of nonspecific recombination in cloned DNAEasy-to-use One Shot formatOmniMAX 2 T1RChemically CompetentE. colicells are supplied in the convenient, single-reaction One Shot format. The single-tube, single-use format allows all steps of the transformation protocol, up to plating, to take place in the same tube, thereby helping save time and prevent contamination.
GenotypeF´ [proAB+lacIqlacZΔM15Tn10(TetR) Δ(ccdAB)]mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 Δ(lacZYA-argF)U169endA1recA1supE44thi-1gyrA96 (NalR)relA1tonApanDFind the strain and format that fit your needsWe offer other strains inchemically competentandelectrocompetent cellformats to meet your specific needs.
OmniMAX 2 T1Rand other strains are available inMultiShot formatsfor high throughput applications.
Explorebacterial growthmedia formats.
Antibiotic Resistance Bacterial: Yes (Tetracycline)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Cloning Unstable DNA: Not suitable for cloning unstable DNA
Contains F' Episome: Yes
High-throughput Compatibility: Low
Improves Plasmid Quality: Yes (endA1)
Plasmid: May be used for plasmids >20 kb
Preparing Unmethylated DNA: No
Product Line: One Shot™
Product Type: Chemically Competent Cells
Quantity: 21 x 50 μL/tube
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency Level: High Efficiency (>1 x 109cfu/μg)
Format: Tube
Species: E. coli (K12)
Unit Size: Each