BRAND / VENDOR: Thermo Fisher

Thermo Fisher, C8581201, MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells

CATALOG NUMBER: C8581201

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Product Description

MultiShot™ FlexPlate OmniMAX™ 2 T1R Competent Cells MultiShot FlexPlate OmniMAX 2 T1Rchemically competentE. colicells are a superior strain of cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity. The FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.
All of our T1Rstrains have thetonA (also known asfhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyseE. colihosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.
Achieve highest transformation efficiency with OmniMAX 2 T1Rchemically competent cellsThe MultiShot FlexPlate OmniMAX 2 T1RE. colistrain is an improved chemically competent cell line that provides transformation efficiencies in the MultiShot FlexPlate format of >5 x 108cfu/μg with control plasmid DNA. It is versatile strain, perfect to use in all cloning applications, including with Gateway technology and TOPO PCR cloning. The OmniMAX 2 T1Rstrain lacks theE. coliK12 restriction systems (mcrA Δ(mrr hsdRMS-mcrBC)) allowing efficient transformation of highly methylated DNA. The OmniMAX 2 T1Rstrain has thelacZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries therecA1 mutation that helps reduce the rate of recombination while propagating plasmid DNA, and theendA1 mutation that increases plasmid DNA yield and quantity. The OmniMAX 2 T1Rstrain contains an F´ episome that can support cloning plasmids with an f1-like origin of replication (phagemids). In addition, the F´ episome carries thelacIq repressor for inducible expression fromtrc,tac, andlacpromoters using IPTG. Importantly, theccdA gene present on the F´ episome is deleted, allowing negative selection of Gateway vectors since the strain is sensitive to theccdB gene product.
One Shot OmniMAX 2 T1RChemically CompetentE. colioffer:
• Highest transformation efficiency in FlexPlate format of >5x108cfu/μg control DNA
•tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Δ(ccdAB) for sensitivity to toxic effects ofccdB gene product, allowing negative selection of vectors containingccdB gene (Gateway cloning system)
• Stable F´ episome that carrieslacIq repressor for inducible expression fromtrc,tac, andlacpromoters
• Construction of more representative genomic libraries due to the elimination ofmcrA,mcrBC,mrr, andhsdRMS
•lacZΔM15 for blue-white color screening of recombinant clones
•endA1 for cleaner DNA preparations and better results in downstream applications due to elimination of nonspecific digestion by Endonuclease I
•recA1 for reduced occurrence of nonspecific recombination in cloned DNAIncrease transformation productivity with MultiShot FlexPlate formatThe FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.
Key benefits of the MultiShot FlexPlate format:
•Increase throughput—compatible with automated liquid handling platforms
•Flexible—transform in increments of 8 wells to entire 96-well plate
•Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformationGenotypeF´ [proAB+lacIqlacZΔM15Tn10(TetR) Δ(ccdAB)]mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 Δ(lacZYA-argF)U169endA1recA1supE44thi-1gyrA96 (NalR)relA1tonApanDFind the strain and format that you needWe offer many otherE. colistrains and formats ofchemically competent cellsandelectrocompetent cellsto help meet your specific transformation needs. If you require other high-throughput transformation options, choose from our collection ofMultiShot formatted comp cellsoremail usfor custom formatting options. We offerbacterial growthmedia in various formats.
Antibiotic Resistance Bacterial: Yes (Tetracycline)
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: Yes (mcrA)
Contains F' Episome: Yes
High-throughput Compatibility: High
Improves Plasmid Quality: Yes (endA1)
Plasmid: May be used for plasmids > 20 kb
Preparing Unmethylated DNA: No
Product Line: Multishot™
Product Type: Chemically Competent Cells
Quantity: 1 x 96 well plate (20 μl/well)
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Format: 96-well Plate
Species: E. coli (K12)
Unit Size: Each

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