Thermo Fisher, C8681201, MultiShot™ FlexPlate Mach1™ T1R Competent Cells

MultiShot™ FlexPlate Mach1™ T1R Competent Cells MultiShot FlexPlate Mach1 T1Rchemically competentE. colicells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity. These chemically competent cells are well suited to reduce the time required for clone evaluation. The convenient FlexPlate format can be divided down to any combination of 8-well segments for flexibility in transformation throughput.
All of our T1Rstrains have thetonA (also known asfhuA) deletion in their genotype that confers resistance to T1 and T5 phage. T1 bacteriophage spread rapidly and lyseE. colihosts, which are commonly used for cloning and library construction. A resistant genotype offers added security to protect valuable clones and libraries. This is especially important for genome and sequencing centers where T1 phage infection would be catastrophic.
Accelerate cloning workflow using Mach1 T1RstrainMultiShot FlexPlate Mach1 T1RcompetentE. colicells are fast growing chemically competent cells with a doubling time of approximately 50 minutes. Colonies are clearly visible within 8 hours of plating the transformation mix (ampicillin selection), enabling for plating and selecting of colonies the same day. Plasmid DNA may also be prepared in four hours after inoculating a single, overnight-grown colony in the selective media of choice (with any antibiotic).
MultiShot FlexPlate Mach1 T1RCompetent Cells offer:
• Transformation efficiencies of >1 x 108cfu/μg plasmid DNA
•tonA (fhuA) genotype to confer resistance to T1 and T5 phage
•lacZ∆M15 for blue-white color screening of recombinants
•hsdR mutation for efficient transformation of unmethylated DNA from PCR applications
•recA1398 mutation for reduced occurrence of homologous recombination in cloned DNA
•endA1 mutation for increased plasmid yield and qualityIncrease transformation productivity with MultiShot FlexPlate formatThe FlexPlate is a 96-well PCR plate that can be used in automated liquid handling systems or divided into 8-well segments for flexibility in transformation throughput. Addition of DNA to the MultiShot FlexPlate Competent Cells can be quickly performed with a multichannel pipet or a liquid handling system. After addition of DNA, the cells can be efficiently transformed by heat-shock using a water bath, heat block, or thermal cycler.
Key benefits of the MultiShot FlexPlate format:
•Increase throughput—compatible with automated liquid handling platforms
•Flexible—transform in increments of 8 wells to entire 96-well plate
•Convenient—competent cells pre-aliquoted into 96-well plate, ready for transformationGenotype:F–φ80lacZΔM15 ΔlacX74hsdR(rK–, mK+) ΔrecA1398endA1tonAFind the strain and format that you needA variety ofE. colistrains and formats ofChemically Competent cellsandElectrocompetent cellsare available to meet specific transformation needs.
We offerbacterial growthmedia in various formats.
For high-throughput transformation options, choose from our collection ofMultiShot formatted comp cellsoremail usfor custom formatting options.
Antibiotic Resistance Bacterial: No
Blue/White Screening: Yes (lacZΔM15)
Cloning Methylated DNA: No
Contains F' Episome: No
High-throughput Compatibility: High
Improves Plasmid Quality: Yes (endA1)
Preparing Unmethylated DNA: No
Product Line: Multishot™
Product Type: Chemically Competent Cells
Quantity: 1 x 96 well plate (20 μl/well)
Reduces Recombination: Yes (recA1)
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Format: 96-well Plate
Species: E. coli (wild-type W)
Unit Size: Each