BRAND / VENDOR: Thermo Fisher

Thermo Fisher, CS10100, ChargeSwitch™ Plasmid ER Mini Kit

CATALOG NUMBER: CS10100

Regular price$0.99
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Product Description

ChargeSwitch™ Plasmid ER Mini Kit The ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA from 1-5 ml of fresh, overnight bacterial cultures without the use of chaotropic salts, organic solvents, or ethanol, to yield up to 20 μg of transfection-grade plasmid DNA in a total time of about 10 minutes after sample preparation.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performanceHigh Quality, High Yield Plasmid DNAThe ChargeSwitch™ Plasmid ER (Endotoxin Reduced) Mini Kit allows rapid and efficient purification of highly pure plasmid DNA fromE. colicells. After preparing cell lysates, the plasmid DNA can be purified from lysates in less than 10 minutes using a novel magnetic bead-based technology that uses magnetic beads conjugated to a unique ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Yields of up to 20 μg plasmid DNA are typically obtained from 1-5 ml of fresh, overnight bacterial cultures without the use of chaotropic salts, ethanol, organics or isopropanol precipitation, making it ideal for mammalian transfection. ChargeSwitch™ Plasmid ER Mini Kit includes Endotoxin Reduction Reagent (ETRR) that substantially lowers the endotoxin levels (less than 50 EU/ μg DNA) in the purified plasmid DNA.
Compatible with MagnaRack™Magnetic RackThe ChargeSwitch™ Plasmid ER Mini Kit requires the use of a magnetic rack. We recommend using theMagnaRack™Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy 'on the magnet' and 'off the magnet' sample processing.
Reliable Performance in a Variety of Downstream ApplicationsThe ChargeSwitch™ Plasmid ER Mini Kit helps ensure reliable performance of the resulting purified plasmid DNA in a variety of downstream applications, including sequencing, cloning, PCR, mammalian cell transfection, bacterial cell transformation,in vitrotranscription, and nucleic acid labeling.
For Research Use Only. Not intended for diagnostic procedures.
Final Product Type: Plasmid DNA
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
Product Line: ChargeSwitch™
Product Type: Plasmid ER Mini Kit
Quantity: 50 Preps
Sample Type: Bacterial Culture
Scale: Mini
Shipping Condition: Room Temperature
Target: Plasmid DNA
Test Time: 10 min.
Format: Kit
Isolation Technology: ChargeSwitch Chemistry
Unit Size: Each

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