Thermo Fisher, EC0113, DH10B Competent Cells

DH10B Competent Cells Thermo Scientific DH10B Competent Cells are high efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently. These cells are ideal for the construction of gene banks or for the generation of cDNA libraries using plasmid-derived vectors. The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal.
• High transformation efficiency: >1x109cfu/μg pUC19 DNA
• Suitable for routine and high-throughput cloning applications
• Allows for blue/white colony screening
• Convenient two reactions per vial packaging
• S.
O.
C. Outgrowth medium includedApplicationsDH10B Competent Cells are suitable for variety of applications requiring high transformation efficiency:
• Efficient DNA cloning both of prokaryotic and eukaryotic origin
• Cloning of large plasmids and BACs
• Ideal for generation of cDNA libraries using plasmid-derived vectors
• Site directed mutagenesisGenotypeF–mcrAΔ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ (ara-leu)7697galU galKλ–rpsL(StrR)nupGNote: Optimized for use with the CloneJET PCR Cloning Kit (Cat. Nos. K1231, K1232) and Phusion Site-Directed Mutagenesis Kit (Cat. No. F541), and available as combo versions (Cat. Nos. K123120, K123240, F542).
Product Type: Competent Cell
Contains F' Episome: Lacks F' Episome
Efficiency: High Efficiency (>1x10^9 cfu/μg pUC19 DNA)
Cell Type: Chemically Competent
Libraries: cDNA
Cloning Methylated DNA: Yes
Bacterial or Yeast Strain: DH10B™
Blue/White Screening: Yes
High-throughput Compatibility: Not High-throughput Compatible
Propagating ccdB Vectors: Not for ccdB vector propagation
Shipping Condition: Dry Ice
Species: E. coli
Format: Tube
Product Line: Thermo Scientific™
For Use With (Application): Cloning
Quantity: 10 x 100 μL
Unit Size: Each