Thermo Fisher, EN0321, S1 Nuclease (100 U/μL)

Thermo Fisher, EN0321, S1 Nuclease (100 U/μL) Thermo Scientific S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides. It is five times more active on DNA than on RNA. S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop. S1 Nuclease exhibits 3'-phosphomonoesterase activity.
The enzyme is a glycoprotein with carbohydrate content of 18%.
Applications
• Removal of single-stranded overhangs of DNA fragments
• S1 transcript mapping
• Cleavage of hairpin loops
• Creation of unidirectional deletions in DNA fragments in conjunction with Exonuclease IIINoteS1 Nuclease can introduce breaks into double-stranded DNA, RNA and DNA/RNA hybrids at high enzyme and low salt concentrations.
Compatible Buffer: Storage Buffer, 5X Reaction Buffer
Product Type: S1 Nuclease
Quantity: 10,000 units
Concentration: 100 U/μL
Enzyme: Nuclease
Unit Size: Each