Thermo Fisher, EN0362, Uracil-DNA Glycosylase (1 U/μL)

Thermo Fisher, EN0362, Uracil-DNA Glycosylase (1 U/μL) Thermo Scientific Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA (seeFigure 1 in Supporting Data). The enzyme shows no activity on RNA.
Highlights
•Activein Thermo Scientific buffers for restriction enzymes and thermophilic polymerasesApplications
• Control of carry-over contamination in PCR
• Glycosylase mediated single nucleotide polymorphism detection (GMPD)
• Site-directed mutagenesis
• As a probe for protein-DNA interaction studies
• SNP genotyping
• Cloning of PCR products
• Generation of single strand overhangs of PCR products and cDNANoteThe abasic sites formed in DNA by Uracil-DNA Glycosylase may be subsequently cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. UDG (UNG) is active in the presence or absence of divalent cations.
Use of this enzyme in certain applications may be covered by patents and may require a license.
Compatible Buffer: 10X Reaction Buffer
Product Type: Uracil DNA Glycosylase
Quantity: 5 x 200 units
Concentration: 1 U/μL
Enzyme: Uracil-DNA Glycosylase
Unit Size: Each