Thermo Fisher, EN0581, Exonuclease I (20 U/μL)

4,000 units Thermo Scientific Exonuclease I (ExoI) degrades single-stranded DNA in a 3'→5' direction. It releases deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaves 5'-terminal dinucleotides intact.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups.
Highlights
• Active in PCR buffersApplications
• Primer removal from PCR mixtures:
• prior to PCR product sequencing
• for one-tube 'megaprimer' PCR mutagenesis
• Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures
• Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminusNoteThe enzyme is not suitable for removing 3'-overhangs of dsDNA.
Product Type: Exonuclease I
Quantity: 4,000 units
Concentration: 20 U/μL
Enzyme: Exonuclease
Unit Size: Each