Thermo Fisher, EP212B6B003, Lyo-ready SuperScript™ III Flash Reverse Transcriptase (60 U/μL)

2000 kU Invitrogen Lyo-ready SuperScript III Flash Reverse Transcriptase joins the SuperScript product family as a reverse transcriptase designed for one-step RT-qPCR and point-of-care assay development. It is a novel SuperScript III Reverse Transcriptase variant, engineered for enhanced speed, thermostability, and inhibitor tolerance. Lyo-ready SuperScript III Flash Reverse Transcriptase provides enhanced sensitivity as low as five copies per reaction.
Benefits of Lyo-ready SuperScript III Flash Reverse Transcriptase include:Excellent choice for one-step RT-qPCRFast cDNA synthesis(~1 minute) reduces the time to resultsHot-startenables high-throughput applications, benchtop stability, and improved specificity of one-step RT-qPCRThermostability up to 70°Callows elevated reaction temperatures and cDNA synthesis from complex RNA targetsHigh inhibitor tolerancemakes the system less dependent on RNA sample purityCommercial rights and customizationenable product usage for commercial purposesHot startLyo-ready SuperScript III Flash Reverse Transcriptase is inactive at ambient temperatures due to the hot start mechanism, which enables room temperature reaction setup and improved specificity and consistency of RT-qPCR results. When used in conjunction with hot start DNA polymerase, such as Lyo-ready Platinum™ II Taq DNA Polymerase, a fully assembled master mix can be stored at room temperature for up to 24 hours, enabling high-throughput applications and compatibility with automation and point-of-care assay development. Due to enhanced specificity, this pair of enzymes also enables multiplex RT-qPCR of up to five targets.
Lyo-readyLyo-ready SuperScript III Flash Reverse Transcriptase is a glycerol-free enzyme that ensures compatibility with microfluidics, air-drying, and lyophilization technologies. For a full lyo-ready one-step RT-qPCR solution, we recommend using SuperScript III Flash Reverse Transcriptase together with Lyo-ready Platinum IITaqDNA polymerase. To learn more about Lyo-ready Enzymes for molecular diagnostics development visit the page thermofisher.
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OEM reverse transcriptaseThis product includes commercial rights and broad customization capabilities. To learn more about OEM and raw materials for molecular diagnostics visit the page thermofisher.
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Detection Method: qPCR, RT-PCR
Format: Stand-alone Enzyme
PCR Method: 1-step RT-qPCR
Reaction Speed: Fast
Optimal Reaction Temperature: 60°C
Quantity: 2000 kU
Ribonuclease H Activity: Reduced
Shipping Condition: Dry Ice
For Use With (Application): Virus Detection
Concentration: 60 U/μL
Hot Start: Build-In Hot Start
Reaction Format: Separate components
Reagent Type: Reverse Transcription
Starting Material: RNA
Unit Size: Each