Thermo Fisher, EPX010-12333-901, CD134 (OX40) Human ProcartaPlex™ Simplex Kit

CD134 (OX40) Human ProcartaPlex™ Simplex Kit The Human CD134 (OX40) Simplex ProcartaPlex Kit measures CD134 (OX40) protein. The kit includes capture beads, detection antibody, and standards.
Human CD134 (OX40) is also included in the Immuno-Oncology Checkpoint 9-Plex Human ProcartaPlex Panel 3 (Cat. No. EPX090-15820-901).
The Simplex kit can be combined with B7-H6, CD276 (B7-H3), CD47 (IAP), CD48 (BLAST-1), CD134 (OX40) ICOS Ligand (B7-H2), TIMD-4, S100A8/A9, and VISTA (B7-H5). When combining multiple Simplex kits, only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.
About ProcartaPlex Assays for the Luminex PlatformProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and just four hours to obtain analyzed results.
More results per sample—measure multiple protein targets simultaneously in a single 25–50 μL sampleWell-established Luminex technology—highly referenced multiplexing platform for protein detection and quantitationProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other bodily fluids.
The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.
g. Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.
The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.
ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit thermofisher.
cn/procartaplex for more information, including a comprehensive list of individual protein targets.
Assay Range: As determined for Lot 1: 7.
52 - 30,800 pg/mL
Assay Sensitivity: As determined for Lot 1: 1,984 pg/mL
Bead Type: CD134 (OX40) [55]
For Use With (Equipment): Luminex™ Instruments
Format: Simplex Kit
Gene: TNF receptor superfamily member 4
Gene Alias: ACT35, OX40, CD134
Gene ID (Entrez): 7293
Gene Symbol: TNFRSF4
Product Line: ProcartaPlex™
Protein: Tumor necrosis factor receptor superfamily member 4
Protein Subtype: ACT35 antigen, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor
Sample Type: Serum, Plasma, Cell Culture Supernatants
Sample Volume: Serum, Plasma: 25 μL, CCS: 50 μL
Shipping Condition: Wet Ice
UniProt ID: P43489
Combinability: Combinable
Product Type: Simplex Kit
Quantity: 96 Tests
Research Area: Immunology, Oncology
Species: Human
Unit Size: Each