Thermo Fisher, ER1401, BseMII (BspCNI) (1 U/μL)

Thermo Fisher, ER1401, BseMII (BspCNI) (1 U/μL) 5'CTCAGN10↓3'3'GAGTCN8↑5'Thermo Scientific BseMII (BspCNI) restriction enzyme recognizes CTCAG(10/8)^ sites and cuts best at 55°C in Tango(+SAM) buffer (isoschizomers: BspCNI). SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificitiesApplications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNPNote:Incubation at 37°C results in 30% activity. Requires SAM for activity. Sinefungin can replace SAM in the restriction reaction. In this case, DNA is not methylated, and more than 95% of the ligated BseMII fragments can be recut by this enzyme. For methylation sensitivity, refer to product specifications.
Compatible Buffer: 10X Buffer Tango
Product Type: Restriction Enzyme
Quantity: 100 units
Concentration: 10 U/μL
Enzyme: BseMII (BspCNI)
Methylation Sensitivity: Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Optimal Reaction Temperature: 55°C
Research Category: Scarless Cloning
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Unit Size: Each