Thermo Fisher, ER1622, LweI (SfaNI) (10 U/μL)

Thermo Fisher, ER1622, LweI (SfaNI) (10 U/μL) 5'GCATCN5↓3'3'CGTAGN9↑5'Thermo Scientific LweI (SfaNI) restriction enzyme recognizes GCATC(5/9)^ sites and cuts best at 37°C in Tango buffer (isoschizomers: SfaNI). SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificitiesApplications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNPNote:LweI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis. At least two copies of LweI recognition site are required for efficient cleavage. For methylation sensitivity, refer to product specifications.
Compatible Buffer: 10X Buffer Tango
Product Type: Restriction Enzyme
Quantity: 500 units
Concentration: 10 U/μL
Enzyme: LweI (SfaNI)
Methylation Sensitivity: CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
Optimal Reaction Temperature: 37°C
Research Category: Scarless Cloning
Sensitive to Heat Inactivation: Yes
Type IIS RE: Yes
Unit Size: Each