Thermo Fisher, K0223, Maxima SYBR Green/ROX qPCR Master Mix (2X)

4,000 Reactions **********************Alternative Product:TryPowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of Maxima SYBR Green qPCR Master Mix and added additional capabilities for your gene expression analysis.**********************Thermo Scientific Maxima SYBR Green qPCR Master Mix is a ready-to-use solution optimized for qPCR and 2-step RT-qPCR. The master mixes include Maxima Hot StartTaqDNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot StartTaqDNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (1). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most most real-time thermal cyclers (seeResources for a compatibility chart).
Highlights
•Specificity—Maxima Hot StartTaqDNA Polymeraseand the optimized buffer eliminate non-specific amplification and formation of primer dimers
•Sensitivity—detects low copy number targets
•Wide linear range—accurate quantification across 9 orders of magnitude
•Reproducibility and convenience—ready-to-use 2x master mixApplications
• Gene expression
• siRNA validation
• Genotyping
• Pathogen detectionIncludesMaxima SYBR Green/ROX qPCR Master Mix (2x) includes Maxima Hot Start Taq DNA Polymerase anddNTPs(alsodUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.
Concentration: 2X
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Format: Tube
No. of Reactions: 4,000 Reactions
Polymerase: Taq DNA Polymerase
Product Line: Maxima
Product Type: Real Time PCR SYBR Master Mix
Quantity: 4,000 reactions
Sample Type: DNA, dsDNA
Sufficient For: 4000 Reactions of 25 μL
Volume: 4 x 12.
5 mL
Detection Method: SYBR
For Use With (Application): Gene Expression
GC-Rich PCR Performance: High
PCR Method: qPCR
Reaction Speed: Standard
Unit Size: Each