Thermo Fisher, K0231, Maxima Probe/ROX qPCR Master Mix (2X)

200 reactions **********************Alternative Product:TryTaqMan Fast Advanced Master Mix, our highest-performance, probe-based master mix. With TaqMan Fast Advanced Master Mix, we’ve taken the best of Maxima Probe qPCR Master Mix and added additional capabilities for your gene expression analysis.**********************Thermo Scientific Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG) (seeReference 1). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.
Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.
Highlights
• Specificity—Maxima Hot Start Taq DNA Polymeraseand the optimized buffer eliminate non-specific amplification and formation of primer dimers
• Sensitivity—detects low copy number targets
• Wide linear range—accurate quantification across 9 orders of magnitude
• Reproducibility and convenience—ready-to-use 2X master mixApplications
• Gene expression
• siRNA validation
• Genotyping
• Pathogen detectionIncludesMaxima Probe/ROX qPCR Master Mix (2X) includes Maxima Hot Start Taq DNA Polymerase and dNTPs (also dUTP) in an optimized PCR buffer supplemented with ROX passive reference dye. Supplied with nuclease-free water.
Concentration: 2X
For Use With (Equipment): 7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus™ System, StepOne™ System
Format: Tube
No. of Reactions: 200 reactions
Polymerase: Taq DNA Polymerase
Product Line: Maxima
Product Type: qPCR Master Mix
Quantity: 200 reactions
Sample Type: DNA, dsDNA
Sufficient For: 200 Reactions of 25 μL
Volume: 2 x 1.
25 mL
Detection Method: Primer-probe
For Use With (Application): Gene Expression
GC-Rich PCR Performance: High
PCR Method: qPCR
Reaction Speed: Standard
Unit Size: Each