Thermo Fisher, K0702, GeneJET PCR Purification Kit

250 Preps Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions. It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.
The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%. Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes. The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning,in vitrotranscription, blotting orin situhybridization.
HighlightsHighly efficient:90 to 100% recoveries in the range of 100 bp to 10 kbFast:procedure takes only 5 minutesConvenient:spin columns are capped and assembled with collection tubesApplicationsFast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including:FastDigestorconventional restrictiondigestionAutomated fluorescent or radioactive sequencingPCRIn vitrotranscriptionIn situhybridizationLabelingCloningElution Volume Max: 50 μL
Final Product Type: DNA
Format: Spin Column
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Isolation Technology: Silica Spin Column
Purification Time: 5 min.
Quantity: 250 Preps
Run Time: 5 min.
Sample Type: PCR Reactions, Enzymatic Reactions (DNase digestions, RNA labeling and clean-up preps, IVT reactions)
Shipping Condition: Room Temperature
Starting Material Volume: ≤400 μL
Yield: 25 μg (Binding capacity)
For Use With (Application): Nucleic Acid Labeling, Southern Blotting, DNA Sequencing, Nucleic Acid Hybridization, Nucleic Acid Hybridization
Unit Size: Each