Thermo Fisher, K0761, GeneJET Whole Blood RNA Purification Mini Kit

GeneJET Whole Blood RNA Purification Mini Kit Thermo Scientific GeneJET Whole Blood RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from whole blood and related body fluids.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 15 minutes. The purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting, and other RNA-based analysis.
HighlightsEfficient– up to 1.
8 μg of total RNA from 500 μL of human bloodConvenient– spin columns are capped and assembled with collection tubesPure– RNA pure from enzyme inhibitors, proteins, ready to use in all demanding applications. Validated in cDNA synthesis and quantitative RT-PCRFast and simple– procedure takes less than 15 minutes and does not involve organic extraction or differential RBC lysisCompatible– suitable for vide variety of samples - human and animal blood treated with EDTA, citrate or heparin, buffy coat, bone marrow, and related body fluidsApplicationsFast extraction of high purity RNA suitable for all conventional molecular biology procedures, including:RT-PCRRT-qPCRNorthern blottingNuclease protection assaysncludes Lysis Buffer Wash Buffer WB 1 (concentrated) Wash Buffer 2 (concentrated) Water, nuclease-free GeneJET RNA Purification Columns (pre-assembled with collection tubes) Collection tubes, 2 mL Collection tubes, 1.
5 mL Detailed ProtocolElution Volume: 50 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Purification Time: 15 min. (After sample homogenization)
Quantity: 50 Preps
Scale: Mini
Shipping Condition: Room Temperature
Starting Material Amount: Blood: 50–500 μL, Bone Marrow: 50–500 μL, Buffy Coat: 500 μL
Yield: Up to 11 μg
Isolation Technology: Silica Spin Column
Sample Type: Blood, Bone Marrow, Buffy Coat
Unit Size: Each