Thermo Fisher, K0781, GeneJET Whole Blood Genomic DNA Purification Mini Kit

50 Reactions Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.
Highlights
•Efficient—high yields of high molecular weight genomic DNA
•Fast—20 minute procedure following cell lysis
•Convenient—spin columns are capped and assembled with collection tubes
•Pure—DNA pure from enzyme inhibitors, RNA and proteins. Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
•Compatible—suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluidsApplications
• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting Includes Proteinase K Solution Lysis Solution Wash Buffer WB I (concentrated) Wash Buffer II (concentrated) Elution Buffer GeneJET Purification Columns (pre-assembled with collection tubes) Collection tubes Detailed ProtocolColumn Type: Spin Column
For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Product Line: GeneJET
Product Type: Genomic DNA Purification Kit
Quantity: 50 Reactions
Sample Type: Blood
Scale: Mini
Target: Genomic DNA
Test Time: <20 min.
Format: Spin Column
Isolation Technology: Spin Column
Unit Size: Each