Thermo Fisher, K0782, GeneJET Whole Blood Genomic DNA Purification Mini Kit

250 Preps Thermo Scientific GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.
Highlights
•Efficient—high yields of high molecular weight genomic DNA
•Fast—20 minute procedure following cell lysis
•Convenient—spin columns are capped and assembled with collection tubes
•Pure—DNA pure from enzyme inhibitors, RNA and proteins. Ready to use for all demanding applications and validated in real-time PCR, endpoint PCR, multiplex PCR, and restriction enzyme digests
•Compatible—suitable for wide variety of samples like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, and body fluidsApplications
• Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting Includes Proteinase K Solution Lysis Solution Wash Buffer WB I (concentrated) Wash Buffer II (concentrated) Elution Buffer GeneJET Purification Columns (pre-assembled with collection tubes) Collection tubes Detailed ProtocolColumn Type: Spin Column
Elution Volume: 50 to 200 μL
Final Product Type: gDNA
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Sample Type: Bone Marrow, Buffy Coat, Dried Blood Spots, Buccal Swab, Urine, Whole Blood
Scale: Mini
Shipping Condition: Room Temperature
Starting Material Amount: Bone Marrow: ≤200 μLBuccal Swabs: 1Buffy Coat: 200 μLDried Blood Spots: variesUrine: ≤4.
5 mLWhole Blood: ≤500 μL
Test Time: 20 min. (After sample homogenization)
Yield: ≤65 μg
Isolation Technology: Silica Spin Column
Unit Size: Each