Thermo Fisher, K0871, GeneJET Stabilized and Fresh Whole Blood RNA Kit

GeneJET Stabilized and Fresh Whole Blood RNA Kit Thermo Scientific GeneJET™ Stabilized and Fresh Whole Blood RNA Kit is designed for rapid and efficient purification of high quality total RNA from various whole blood samples: fresh, frozen, or human blood samples stabilized with commercial stabilizers such as Tempus™ and PAXgene™.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The kit includes a DNase l for the enzymatic removal of genomic DNA. RNA can be isolated from whole blood in less than 30 minutes (including cell lysis and DNase l treatment).
HighlightsVersatile—compatible with fresh and frozen blood, buffy coat, as well as blood collected in PAXgene™and Tempus™ TubesConvenient—specially optimized Lysis Buffer is suitable for RNA stabilization up to 48 hEfficient—up to 4 μg of total RNA from 500 μL of healthy human bloodPure—isolates intact total RNA free of any RT-qPCR inhibitorsApplicationsIsolated total RNA can be used in:RT-PCRRT-qPCRNorthern BlottingIncludesLysis Buffer (R2841)Wash Buffer 1 (conc.) (R2851)Wash Buffer 2 (conc.) (R2861)DNase l (lyophilized)DNase I Reconstitution Buffer2x DNase I BufferManganese Chloride SolutionWater, nuclease-freeGeneJET RNA Purification Columns pre-assembled with Collection TubesCollection Tubes, 2 mLCollection Tubes, 1.
5 mLConcentration: 2X
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Nucleic Acid Labeling, Northern Blotting, cDNA Library Construction
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 50 Preps
Product Line: GeneJET
Purification Time: 30 min.
Quantity: 50 preps
Format: Spin Column
Isolation Technology: Spin Column
Sample Type: Blood
Target: Total RNA
Unit Size: Each