Thermo Fisher, K1138, ToxBLAzer™ DualScreen Kit

200 μg ToxBLAzer™ DualScreen Kit provide the key advantage of combining the ratiometric reporter gene readout of beta-lactamase with a cytotoxicity readout in the same assay. Thus, ToxBLAzer™ DualScreen yields reporter readouts that normalize cytotoxic cellular responses, which is a necessity in screening applications which down regulate transcription. The ToxBLAzer™ substrate contains both the LiveBLAzer™-FRET B/G substrate and a proprietary cytotoxicity indicator. Both compounds readily enter the cell, where endogenous esterases rapidly convert them into fluorescent compounds Esterase activity and an intact cell membrane are required for detection of the indicator. The cytotoxicity indicator's fluorescence is red-shifted to ensure compatibility with the blue and green emissions of the LiveBLAzer™-FRET B/G substrate, allowing detection of both reporter gene activity and cytotoxicity in the same assay sample.
Advantages of ToxBLAzer™ DualScreen Kits:
• Multiplexed readout results in rapid identification of false positives due to toxicity or lack of viable cells, thereby improving hit-picking efficiency
• Ratiometric blue:green reporter readout reduces false hits by minimizing experimental noise
• Single reagent addition eliminates need for follow up cytotoxicity assays, thereby increasing throughput while reducing sample and test compound consumptionCell Permeability: Cell-Permeant
Detection Method: Fluorescence
For Use With (Equipment): Microplate Reader, Fluorometer, Flow Cytometer
Format: Kit
Product Type: Dual Screen Kit
Quantity: 200 μg
Substrate: Beta-Lactamase Substrate
Product Line: ToxBLAzer™
Unit Size: Each