Thermo Fisher, K1423, Rapid DNA Ligation Kit

150 Reactions Thermo Scientific Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature. The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with theTransformAid Bacterial Transformation Kitor with other conventional transformation procedures. Highlights
• Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes
• Convenient—reaction mixture can be used directly for bacterial transformationApplications
• Routine cloning experiments
• Blunt-end cloning
• Library construction
• TA cloningIncludes
•T4 DNA Ligase
• 5X Rapid Ligation Buffer
•Water, nuclease-free
• Detailed ProtocolNotePrior to electroporation, it is necessary to inactivate T4 DNA ligase by chloroform extraction or spin column purification, e. g. withThermo Scientific GeneJET PCR Purification Kit(K0701). Enzyme: DNA Ligase
For Use With (Application): Nucleic Acid Gel Electrophoresis
Product Type: Rapid DNA Ligation Kit
Quantity: 150 Reactions
Sample Type: DNA
Format: Kit
Unit Size: Each