Thermo Fisher, K156001, PureLink™ Total RNA Blood Kit

PureLink™ Total RNA Blood Kit PureLink™ Total RNA Blood Kit allows purification of high-quality total RNA from 50 - 500 μl fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytesSimple RNA Purification from Whole BloodPureLink™ Total RNA Blood Kit is designed for purifying from 50 to 500 μl fresh whole blood or approximately 3.
5 x 106leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.
Leukocyte Enrichment for Improved RNA PurificationWhole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm3) and leukocytes, with the latter present in the minority (5000 - 7000/mm3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink™ Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink™ Total RNA Blood Kit.
Purified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
•Northern Blotting Kits & Reagents
•Nuclease protection assays
•Microarray
• cDNA synthesis
•Real-Time PCR (qPCR)For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
Elution Volume: 30–100 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Purification Time: 20 min. (After sample homogenization)
Quantity: 50 preps
Scale: Mini
Shipping Condition: Room Temperature
Starting Material Amount: Blood: ≤500 μL
Yield: 1000 μg (Binding capacity)
Isolation Technology: Silica Spin Column
Sample Type: Blood
Unit Size: Each