Thermo Fisher, K180001, Easy-DNA™ gDNA Purification Kit

Easy-DNA™ gDNA Purification Kit The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle up to 1 gram of mammalian tissue. Other samples tested include mouse tails, plant leaves, yeast, andE. colicells. These samples yield high-quality DNA with an average size between 100 kb and 200 kb, which is suitable for PCR, DNA hybridization, genomic DNA library construction, and other applications. Advantages of the Easy-DNA™ Kit include:
•Reliability—high-quality genomic DNA from large or small samples of cells, tissue, plant, yeast,E. coli, blood, and even hair follicles
•Simplicity—for most sample types, only 4 steps, typically requiring less than 90 minutes are needed to isolate high-quality DNA
•Versatility—one kit is supported by optimized protocols for a variety of cell and tissue typesSimple workflow, quality resultsOptimized protocols for a variety of cell and tissue types are included in the instruction manual supplied with the kit. Generally, small-scale gDNA preparations are performed in microfuge tubes, in which samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After extraction with chloroform (not included in kit), precipitation, and reconstitution of the DNA pellet, the preparation is complete. The kit contains a protein degradation reagent (for use with high-protein–containing samples such as connective tissue), mussel glycogen for use as a co-precipitant for dilute DNA samples, and an RNase solution.
Elution Volume: 10 to 150 μL
Final Product Type: gDNA
For Use With (Application): PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 1 Kit
Sample Type: Bacteria, Cells, Hair Follicles, Mouse Tails, Plant, Tissue, Virus, Whole Blood, Yeast
Shipping Condition: Room Temperature
Starting Material Amount: Bacteria: ≤109cellsCells: ≤108Hair Follicles: ≤5Mouse Tails: 1 cmPlant: ≤50 mgTissue: ≤1 gVirus: ≤750 μLWhole Blood: ≤2 mLYeast: ≤10 mL
Test Time: Up to 90 min.
Yield: ≤850 μg
Isolation Technology: Organic Extraction
Unit Size: Each