Thermo Fisher, K182000, PureLink™ Genomic DNA Mini Kit

10 Preps The PureLink™ Genomic DNA Mini Kit enables high-yield, high-purity, genomic DNA (gDNA) extractions from a wide variety of sample types. Use this single kit for genomic DNA purification from blood, tissues, cells, bacteria, swabs, and blood spots, with a familiar silica-based, microcentrifuge spin-column format. Features of the PureLink™ Genomic DNA Mini Kit include:
•Kit flexibility—one kit works with a variety of sample types and sizes
•Ultraclean gDNA—minimal contamination of DNA product means successful downstream application
•Improved design—optimized spin-column design and buffer formulation for better yield and purityMultiple sample sources, one kitThe PureLink™ Genomic DNA Mini Kit can be used with many different sample types, each with its own optimized protocol outlined in the manual.
Optimized components and protocolsThe PureLink™ Genomic DNA Mini Kit includes parts and protocols that improve upon the easy and familiar spin-column methods currently in use (see figure). Columns are newly configured to bind and release DNA more efficiently (see figure). Each sample source uses a specialized lysis procedure and an optimized lysis buffer formulated to enhance proteinase K activity and eliminate protein contamination. A chaotropic salt buffer promotes stable binding of DNA to the column resin, and powerful wash buffers remove all traces of protein and salt. DNA is eluted in a low-salt buffer to allow for pH stabilization of the DNA in storage.
Higher yields and purityWith PureLink™ Genomic DNA Kits you can expect high yields of high-purity gDNA (determined by A260/A280measurements), no matter the sample type, from bacteria to tissues to blood to cultured cells (see figures). The enhanced PureLink™ Lysis and Wash Buffers help to consistently deliver purified gDNA samples with absorbance reading ratios of ∼1.
9 for both A260/A280and A260/A230, indicating no detectable contamination from carryover of protein or guanidine (see figure).
For Use With (Application): Genotyping, Real-Time Quantitative PCR (qPCR), Southern Blotting, Sequencing, PCR
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: 100 μL to 1 mL Blood Samples (Medium-Scale)
Product Line: PureLink™
Product Type: Genomic DNA Mini Kit
Quantity: 10 Preps
Sample Type: Bacteria, Blood, Cells, Tissue
Scale: Mini
Shipping Condition: Room Temperature
Target: Genomic DNA
Isolation Technology: Silica Spin Column
Unit Size: Each