Product Description
Thermo Fisher, K191001, S.
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P.™ Plasmid DNA MidiPrep Kit The S.
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P.™ MidiPrep Kit is a medium-scale plasmid DNA purification kit designed for fast and easy purification of pure plasmid DNA from 10-100 ml of overnight culture (Figure 1). The S.
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P.™ MidiPrep procedure takes just 40 minutes. You simply:
• Lyse cells and filter debris with the S.
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P.™ filtering column
• Apply flow-through to the S.
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P.™ DNA binding column
• Wash the column
• Elute pure, supercoiled plasmid DNA in water or TE bufferPlasmid DNA purified with the S.
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P.™ MidiPrep Kit is ready to use right off the column. No precipitation is necessary. Plasmid is eluted in TE or water at ∼200 ng/μl-perfect for subsequent applications including mammalian cell transfection. Typical yields are shown in Table 1.
Final Product Type: Plasmid DNA
For Use With (Application): Sequencing, Cloning, Transformation, Transfection, In Vitro Transcription
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Prep Scale: 100-200 μg (Medium-Scale) Plasmid DNA
Product Line: S.
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P. ™
Product Type: Plasmid DNA MidiPrep Kit
Quantity: 20 preps
Sample Type: Bacterial Culture
Scale: Midi
Target: Plasmid DNA
Test Time: 40 min.
Format: Kit
Isolation Technology: Spin Column
Unit Size: Each
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Collaboration
Tony Tang
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