Thermo Fisher, K210002, PureLink™ HiPure Plasmid Miniprep Kit

25 Preps The PureLink™ HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA fromE.
coliin less than 2 hours, without the use of any organic solvents or cesium chloride, and contains low endotoxin levels.
• Fast—Highly-quality purified plasmid DNA in under 2 hours
• Efficient—Increased yields with low endotoxin levelsPlasmid DNA of High PurityThe PureLink™ HiPure Plasmid Miniprep Kit is designed to efficiently isolate plasmid DNA fromE.
coliin 1.
5-2 hours. The kit employs anion-exchange resin composed of small particles with a uniform pore size to purify plasmid DNA to a level equivalent to two passes through CsCl gradients (Figure 1). The need for additional steps to remove contaminants such as phenol, chloroform, and CsCl are eliminated, minimizing exposure to and disposal of hazardous materials.
High Yield and Efficient Endotoxin RemovalPureLink™ HiPure Plasmid Miniprep Kit allows purification of up to 30 μg of high quality plasmid DNA from 1-3 mL overnightE.
colicultures in approximately 1 hour, with an endotoxin level of 0.
1-1 EU/ μg, when cloning high copy number plasmids (Table 1). Typically, DNA isolated using the PureLink™ HiPure Plasmid Miniprep Kit has an A260/A280> 1.
80 when samples are diluted in Tris-HCl (pH 7.
5) indicating that the DNA is reasonably free of proteins that could interfere with downstream applications.
Isolated DNA can be used for a Number of Downstream Applications:The purified DNA is ultrapure and suitable for downstream applications, including those requiring the highest purity DNA, such as mammalian transfections (Figure 2), automated and manual DNA sequencing, PCR amplification,in vitrotranscription, bacterial cell transformation, cloning, and labeling.
For Research Use Only. Not intended for diagnostic procedures.
Final Product Type: BAC DNA, Plasmid DNA
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 25 Preps
Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
Product Line: PureLink™
Product Type: Plasmid MiniPrep Kit
Quantity: 25 Preps
Sample Type: Bacterial Culture
Scale: Mini
Shipping Condition: Room Temperature
Target: BAC DNA, Plasmid DNA
Test Time: 2 hr.
Format: Kit
Isolation Technology: Anion Exchange Resin
Unit Size: Each