Product Description
25 Preps The PureLink™ HiPure Plasmid Maxiprep Kit is designed to isolate transfection-grade plasmid DNA fromE. coli. The Maxiprep Kit protocol will typically yield 500–850 μg plasmid DNA from 100–200 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients.
Advantages of the PureLink™ HiPure Plasmid Maxiprep Kit include:
•Labor and time saving—no need for extra steps to remove endotoxin and other contaminants
•High-purity, low-endotoxin product—pure enough for mammalian cell transfection
•Versatility—purify any type and size of plasmid DNA, including BAC, bacmids, and ssM13 DNAsAnion-exchange chromatography for plasmid purificationThe PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl gradients. The resin combines excellent capacity with a fast flow rate, high resolution, high yield, and efficient endotoxin removal. Plasmid preparations can typically be completed in less than 2 hours.
Contaminant-free DNAUnlike cesium chloride gradient protocols, PureLink™ HiPure Plasmid Purification Systems do not use organic solvents, ethidium bromide, or cesium chloride, all of which are troublesome to work with and to dispose of. Typically, plasmid DNA prepared using the PureLink™ HiPure Plasmid Midiprep Kit will have an A260/A280ratio of >1.
80, indicating that the DNA is reasonably free of proteins that might interfere with downstream applications. Endotoxin levels are typically within 0.
1–1 EU/μg, making this plasmid DNA ideal for mammalian cell transfection.
Elution Volume: 15 mL
Final Product Type: BAC DNA, Plasmid DNA
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 25 Preps
Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
Product Line: PureLink™
Product Type: Plasmid MaxiPrep Kit
Purity: Transfection Grade
Quantity: 25 Preps
Sample Type: Bacterial Culture
Scale: Maxi
Shipping Condition: Room Temperature
System Type: PureLink™
Target: BAC DNA, Plasmid DNA
Test Time: 2 hr.
Yield: 1000 μg
Format: Column
Isolation Technology: Anion Exchange Resin
Unit Size: Each
Order Guidelines
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2. Please DO NOT make payment before confirmtaion.
3. Minimum order value of $1,000 USD required.
4. 100% prepayment required.
Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924