Thermo Fisher, K210017, PureLink™ HiPure Plasmid Filter Maxiprep Kit

25 Preps The PureLink™ HiPure Plasmid Filter Maxiprep Kit is designed to isolate plasmid DNA of the highest purity from 100 to 500 mL of an overnight bacterial culture. The PureLink™ HiPure Plasmid Filter Kits include HiPure Filter Columns, which combine filtration for clarification of the bacterial lysate with anion-exchange resin purification of plasmid DNA, in a single unit. The resulting plasmid DNA is of a quality equivalent to that obtained by purification with two passes through cesium chloride gradients—the most rigorous method for DNA purification. Purify high-quality DNA in less than two hours for transfections and other applications with no need for additional steps to remove contaminants like RNA, proteins, or endotoxins. Additionally, the elimination of phenol, chloroform, ethidium bromide, and cesium chloride from the protocol minimizes exposure to, and disposal of hazardous materials. The PureLink™ HiPure Plasmid Filter Kits provide:
•Simple protocols—bacterial lysate clarification without centrifugation
•Fast results—save time by purifying plasmid DNA in less than two hours
•High yields—up to 200 μg for midipreps and up to 850 μg for maxipreps (see table)
•Reliable performance—the high-quality plasmid DNA purified with these kits is suitable for a wide variety of applicationsHow it worksThe addition of a HiPure Filter Column to the PureLink™ HiPure Plasmid Kits provides rapid clearing of the bacterial lysate without the need for centrifugation (see figure). The lysate filtration cartridge is integrated into the DNA-binding column for a combined step of lysate clarification and plasmid DNA binding directly to the anion-exchange resin. A single column wash removes impurities such as RNA, proteins, metabolites, and other low molecular weight molecules; the ultrapure plasmid DNA is then eluted with a high-salt buffer. The DNA is then desalted and concentrated with an isopropanol precipitation step, then collected by centrifugation. The entire protocol can be completed in about two hours.
Column Type: Filter Column
Elution Volume: 15 mL
Final Product Type: Plasmid DNA
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 25 Preps
Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
Product Line: PureLink™
Product Type: Plasmid Filter MaxiPrep Kit
Purity: Transfection Grade
Quantity: 25 Preps
Sample Type: Bacterial Culture
Scale: Maxi
Shipping Condition: Room Temperature
System Type: PureLink™
Target: Plasmid DNA
Test Time: 90 min.
Yield: 1000 μg
Format: Column
Isolation Technology: Anion Exchange Resin
Unit Size: Each