Thermo Fisher, K210027, PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit

25 Preps The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour. Using the HiPure Filter Columns and HiPure Precipitators supplied in the kit, you can purify plasmid DNA without the centrifugation steps required in traditional isolation protocols. The quality of the purified plasmid DNA is equivalent to that obtained by two passes through cesium chloride gradients - the most rigorous method for DNA purification - making it suitable for transfection and other applications requiring high-purity plasmid DNA.
The PureLink™ HiPure Plasmid FP Maxiprep Kit (Table 1) provides:
• Simple protocols—bacterial lysate clarification and isopropanol precipitation without centrifugation
• Fast results—fromE. colicell pellet to high-purity, transfection-grade plasmid DNA in about 60 minutes
• High yields—up to 850 μg from a high-copy plasmidHow it worksThe PureLink™ HiPure Plasmid FP Maxiprep Kit employs an alkaline lysis method to prepare and rapidly clear bacterial lysates without the need for centrifugation (Figure 1). The unique HiPure Filter Column includes a lysate filtration cartridge integrated into the anion-exchange column for single-step lysate clarification and plasmid DNA binding. Impurities such as RNA, proteins, and other contaminants are removed with a single wash, and ultrapure plasmid DNA is eluted with a high-salt buffer. To precipitate and desalt the DNA, isopropanol is added to the eluted DNA and the mixture is then applied to the HiPure Precipitator using a large syringe. After a subsequent washing and drying step, the plasmid DNA is easily eluted from the HiPure Precipitator with either TE buffer or water. The use of the precipitator eliminates the need for centrifugation, reducing the risk of losing the DNA pellet during supernatant removal, and saving time. The resulting DNA is ready to use in the most challenging applications. The entire protocol - fromE. colicell pellet to ultrapure transfection-grade plasmid DNA - can be completed in about an hour.
Highest yields and ready-to-use concentrationsThe HiPure Precipitator has been optimized to allow for lower elution volumes (down to 500 μL) to produce highly concentrated DNA without a significant decrease in yields. In fact, yields at higher concentrations are consistently higher than the competitor kits (Figure 2). Yields and concentration can be adjusted to meet the needs of your downstream application.
Column Type: Filter Column
Elution Volume: 15 mL
Final Product Type: Plasmid DNA
For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 25 Preps
Plasmid: <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Prep Scale: > 200 μg (Large-Scale) Plasmid DNA
Product Line: PureLink™
Product Type: Plasmid FP MaxiPrep Kit
Purity: Transfection Grade
Quantity: 25 Preps
Sample Type: Bacterial Culture
System Type: PureLink™
Target: Plasmid DNA
Test Time: 1 hr.
Yield: 1000 μg
Format: Column
Isolation Technology: Anion Exchange Resin
Unit Size: Each