Thermo Fisher, K210096, PureLink™ 96 HQ Plasmid DNA Purification Kit

PureLink™ 96 HQ Plasmid DNA Purification Kit The PureLink HQ 96 Plasmid DNA Purification Kit isdesigned for high-throughput isolation of high-qualityplasmid DNA from bacterial cells. It can beused with a vacuum manifold or a centrifuge and iscompatible with automated liquid handling workstations.
The PureLink HQ 96 Plasmid DNA Purification Kit offersthe following advantages:
• Ability to isolate up to 10 μg plasmid DNA per well
• Designed to isolate high-quality plasmid DNA in30-45 minutes
• Minimal genomic DNA contamination in the purifiedplasmid DNA
• Compatible with automated liquid handlingworkstations
• Reliable performance of the purified plasmid DNA indownstream applications such as mammaliantransfectionProcess overviewBacterial cells are harvested and resuspended in ResuspensionBuffer with RNase. The cells are lysed using an alkaline/SDScell lysis procedure. The lysate is then neutralized andconditions are adjusted for subsequent binding. After lysateclarification using the PureLink HQ 96 Clarification Plate, thelysate is processed through the PureLink HQ 96 BindingPlate. The DNA binds to the silica-based membrane in theplate and impurities are removed by washing with WashBuffer. The DNA is then eluted in low salt Elution Buffer orwater.
Final Product Type: Plasmid DNA
For Use With (Application): PCR, Transfection, Sequencing, Transformation, Cloning
For Use With (Equipment): Automated Liquid Handling Workstation
High-throughput Compatibility: High-throughput Compatible
No. of Reactions: 4 x 96 Preps
Prep Scale: < 100 μg (Small-Scale) Plasmid DNA
Product Line: PureLink™
Product Type: Plasmid DNA Purification Kit
Quantity: 4 x 96 preps
Sample Type: Bacterial Culture
Shipping Condition: Room Temperature
Target: Plasmid DNA
Test Time: 30 to 40 min.
Format: 96-well Plate
Isolation Technology: Silica Plate
Unit Size: Each