Thermo Fisher, K440001, pTrcHis2 TOPO™ TA Expression Kit

pTrcHis2 TOPO™ TA Expression Kit The pTrcHis- and pTrcHis2-TOPO™ TA Expression Kits allow fast, efficient cloning of PCR products into a prokaryotic expression vector for high-level, regulated expression from thetrcpromoter. The vector in each kit, pTrcHis-TOPO™ or pTrcHis2-TOPO™, includes a minicistron element for enhanced translation efficiency of eukaryotic proteins inE. coli. Both vectors are provided linearized and activated with topoisomerase I for 5-minute TOPO™ Cloning with >85% cloning efficiency. To simplify protein purification and detection, the pTrcHis-TOPO™ and pTrcHis2-TOPO™ vectors include the following features:pTrcHis-TOPO™
•N-terminal Xpress™ epitope for detection with an Anti-Xpress™ Antibody
• N-terminal polyhistidine (6xHis) tag for purification using nickelchelating resin and detection with an Anti-HisG Antibody
• Enterokinase cleavage site for efficient removal of N-terminal fusion tagspTrcHis2-TOPO™
•C-terminalc-mycepitope for detection with an Anti-mycAntibody
• C-terminal polyhistidine (6xHis) tag for purification using nickel-chelating resin and detection with an Anti-His(C-term) AntibodyAntibiotic Resistance Bacterial: Ampicillin (AmpR)
Bacterial or Yeast Strain: TOP10
Cleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Inducible
Expression Mechanism: Cell-Based Expression
Expression System: E. coli
Inducing Agent: IPTG
Product Type: TA Expression Kit
Quantity: 20 reactions
Selection Agent (Eukaryotic): None
Vector: pTrc
Cloning Method: TOPO-TA
Product Line: TOPO™
Promoter: Trc, lacO
Protein Tag: His Tag (6x), c-Myc Epitope Tag
Unit Size: Each