Product Description
InsectSelect™ Glow Kit with Sf9 Cells The InsectSelect™ Glow Kit features the pIZT/V5-His vector for high-level expression of yourgene of interest. This vector has the following features:
• TheOpIE2 promoter for constitutive expression
• The Zeocin™ resistance gene for rapid selection of stably transfected cell lines
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resinIn addition, pIZT/V5-His expresses a fusion of the green fluorescent protein and the Zeocin™ resistanceprotein (Zeo-GFP). The Zeo-GFP fusion protein permits rapid selection of stably transfected cell lineswith Zeocin™ and confers a fluorescent phenotype that simplifies identification of transfected cells.
Product Type: Sf9 Cells Glow Kit
Protein Tag Position (to your gene): C-terminal
Quantity: 1 kit
Vector: pIZ
Cloning Method: Restriction Enzyme/MCS
Product Line: InsectSelect™
Promoter: OplE2
Protein Tag: His Tag (6x), V5 Epitope Tag
Unit Size: Each
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Collaboration
Tony Tang
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