Thermo Fisher, K830001, pcDNA™3.3-TOPO™ TA Cloning™ Kit

Thermo Fisher, K830001, pcDNA™3.
3-TOPO™ TA Cloning™ Kit The pcDNA™3.
3-TOPO™ TA Cloning™ Kit is designed to deliver exceptionally high levels of transgene expression in adherent cells as well as in the FreeStyle™ MAX CHO and FreeStyle™ MAX 293 suspension cell systems. The pcDNA3.
3™-TOPO™ TA Cloning™ Kit is supplied with the TOPO™-adapted plasmid vector, all reagents for cloning, and One Shot™ TOP10 competent cells. The pcDNA™3.
3-TOPO™ vector offers the following benefits:
• TOPO™ adapted for cloning PCR products with >85% efficiency
• High-level gene expression in a wide range of mammalian cells due to the native cytomegalovirus (CMV) immediate-early promoter/enhancer sequence (672 bp)
• Vector is ideal for large-scale protein expression
• Generation of native protein without extraneous amino acidsThe pcDNA™3.
3-TOPO™ vector is also included with the OptiCHO™ Antibody Express System for cloning and expression of light and heavy antibody chains.
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
For Use With (Application): Protein Production, Constitutive Expression
Product Type: TOPO TA Cloning Kit
Quantity: 20 reactions
Selection Agent (Eukaryotic): Geneticin™ (G-418)
Vector: pcDNA
Cloning Method: TOPO-TA
Product Line: TOPO™, pcDNA™
Promoter: CMV
Protein Tag: Untagged
Unit Size: Each