Thermo Fisher, K89020, pIB/V5-His TOPO™ TA Expression Kit

pIB/V5-His TOPO™ TA Expression Kit The pIB/V5-His TOPO™ TA Expression Kit offers five-minute cloning ofTaq-amplified PCR products directly into the pIB/V5-His-TOPO™ expression vector. In addition to being TOPO™ Cloning ready, pIB/V5-His-TOPO™ includes:
• TheOpIE2 promoter for constitutive expression
• The Blasticidin resistance gene for rapid selection of stably transfected cell lines in two weeks
• C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification using nickel-chelating resinProduct Type: TOPO TA Expression Kit
Protein Tag Position (to your gene): C-terminal
Quantity: 20 reactions
Vector: pIB, TOPO-TA Cloning Vectors
Cloning Method: TOPO-TA
Product Line: InsectSelect™, TOPO™
Promoter: OplE2
Protein Tag: His Tag (6x), V5 Epitope Tag
Unit Size: Each