Thermo Fisher, K961020, pEF6/V5-His TOPO™ TA Expression Kit

Thermo Fisher, K961020, pEF6/V5-His TOPO™ TA Expression Kit The pEF6/V5-His TOPO™ TA Expression Kit is designed for high-level mammalian expression from the powerful human EF-1 α promoter with the added convenience of one-step TOPO™ Cloning. The pEF6/V5-His-TOPO™ vector includes:Enhancer/promoter elements from the human elongation factor 1α subunit (hEF-1α) for high-level expression in mammalian cells
• The Blasticidin resistance gene for rapid selection of stable mammalian cell lines
• A C-terminal V5 epitope for efficient detection with an Anti-V5 Antibody
• A C-terminal polyhistidine (6xHis) sequence for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term)
• AntibodyCleavage: EK (Enterokinase) Recognition Site
Constitutive or Inducible System: Constitutive
Delivery Type: Transfection
For Use With (Application): Constitutive Expression
Product Type: TOPO TA Expression Kit
Quantity: 20 reactions
Selection Agent (Eukaryotic): Blasticidin
Vector: pEF, TOPO-TA Vectors
Cloning Method: TOPO-TA
Product Line: TOPO™
Promoter: EF-1α
Protein Tag: His Tag (6x), V5 Epitope Tag
Unit Size: Each