Thermo Fisher, L3224, LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells Invitrogen™

LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells Invitrogen™ The LIVE/DEAD® Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population based on plasma membrane integrity and esterase activity. The kit can be used in flow cytometry, fluorescence microscopy, and with fluorescence microplate readers.
Advantages Over Alternative Methods Include:
•  Faster
•  Safer
•  More sensitive
•  Less expensiveEasily Use with a Wide Variety of Techniques and Cell TypesUbiquitous intracellular esterase activity and an intact plasma membrane are distinguishing characteristics of live cells. The LIVE/DEAD® Viability/Cytotoxicity Kit quickly discriminates live from dead cells by simultaneously staining with green-fluorescent calcein-AM to indicate intracellular esterase activity and red-fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. It is adaptable to most eukaryotic cells where cytotoxic conditions produce these cellular effects. The assay is useful with a variety of fluorescence-detection methodologies.
Sensitive, Safe, and EfficientThe LIVE/DEAD® Viability/Cytotoxicity Kit is more sensitive than Trypan blue exclusion, a commonly used method for live/dead cell discrimination. The cost-effective LIVE/DEAD® Viability/Cytotoxicity Kit is highly sensitive due to the bright fluorescence of both dyes upon interacting with either live (for calcein-AM) or dead (for ethidium homodimer-1) cells. Background levels are low due to the fact that both dyes are virtually non-fluorescent prior to interacting with cells.
LIVE/DEAD® Assays Available for a Broad Range of ApplicationsA selection of Invitrogen LIVE/DEAD® Viability Assays is offered for mammalian cells, bacteria, yeast and fungi, as well as Fixable Dead Cell Stain Kits for use in intracellular staining for flow cytometry. All LIVE/DEAD® assays provide quick, positive discrimination between viable and non-viable cells.
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Cell Type: Mammalian Cells
Description: LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Detection Method: Fluorescence
Dye Type: Other Label(s) or Dye(s)
Format: Tube(s), 96-well plate, Slide(s)
Quantity: 1 kit
Shipping Condition: Room Temperature
Color: Green, Red
Emission: 517/617
Excitation Wavelength Range: 494, 528 nm
For Use With (Application): Viability Assay
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product Line: LIVE/DEAD
Product Type: Viability/Cytotoxicity Kit
Unit Size: Each