Thermo Fisher, L34952, LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

Thermo Fisher, L34952, LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry TheLIVE/DEAD™FungaLight™ Yeast Viability Kitenables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.
Any physiological buffer between pH 7.
0–8.
0, including PBS, can be used to dilute the SYTO dyes for the staining solution.
Cell Permeability: Impermeant, Permeant
Description: LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry
Detection Method: Fluorescence
Dye Type: SYTO™ 9, propidium iodide
Form: Solution
Format: Tube(s)
Quantity: 200 Assays kit
Shipping Condition: Room Temperature
Color: Green, Red
Emission: 498/617
Excitation Wavelength Range: 485/535 nm
For Use With (Application): Viability Assay
For Use With (Equipment): Flow Cytometer
Product Line: FungaLight, LIVE/DEAD
Product Type: Yeast Viability Kit
Unit Size: Each