Thermo Fisher, M1631, RevertAid™ Master Mix

Thermo Fisher, M1631, RevertAid™ Master Mix Thermo Scientific RevertAid Master Mix is a complete system for efficient synthesis of first-strand cDNA from mRNA or total RNA templates. The mix includes RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase. RevertAid Master Mix provides all cDNA synthesis reaction components in a convenient two-tube master mix. It is optimized for highly efficient cDNA synthesis for two-step quantitative RT-PCR (RT-qPCR) applications. The two-tube formulation has a shorter reaction time and requires fewer pipetting steps than the RevertAid RT Reverse Transcription Kit, while maintaining high performance.
Highlights
• Fast—short reaction time (typically 15–30 min)
• Convenient—easy workflow with few pipetting steps
• Reliable—reverse transcription of both abundant and limited targets
• Flexible—optimized 2-step protocol, enabling multiple PCR reactions from a single reverse-transcription reactionApplicationsFirst strand cDNA synthesis for RT-PCR and RT-qPCR
• Construction of full length cDNA libraries
• Antisense RNA synthesisFormat: Master Mix
Reaction Speed: 30 min.
Technique: Reverse Transcription
Quantity: 50 reactions
Reverse Transcriptase: RevertAid
Ribonuclease H Activity: Yes
Shipping Condition: Dry Ice
For Use With (Application): Real Time PCR (qPCR)
Final Product Type: First-Strand cDNA
No. of Reactions: 50 Reactions
Reaction Format: Premixed Components
Reagent Type: Reverse Transcription
Starting Material: RNA
Unit Size: Each