Product Description
MethylCode™ Bisulfite Conversion Kit The MethylCode™ Bisulfite Conversion Kit is a fast and easy way to convert unmethylated cytosines from a DNA sample into uracils while methylated cytosines remain unchanged.
The modified DNA is ideal for PCR amplification for downstream analyses, including restriction endonuclease digestion, sequencing, and microarrays. With the MethylCode™ Bisulfite Conversion Kit you can expect:–Simple, rapid cytosine methylation detection–Consistency across most experimental conditions–Streamlined conversion of unmethylated cytosines into uracil, thanks to a coupled DNA denaturation/bisulfite treatment step–Ultra-pure DNA ideal for subsequent molecular-based analysesKit specifications:–Protocol: Rapid heat denaturation of DNA followed by sodium bisulfite modification with spin column purification–Samplesources: Plasmid, genomic, and endonuclease digested DNAs–InputDNA: 500 pg-2 μg DNA per treatment, with 200-500 ng being optimal–Recoveryvolume: Purified, bisulfite-treated DNA is recovered in 10 μl elution bufferContents and storage:The MethylCode™ Bisulfite Conversion Kit contains all the reagents necessary to convert your DNA for methylation studies. The kit includes CT Conversion Reagent, Dilution Buffer, Resuspension buffer, Binding Buffer, Wash Buffer, Desulphonation Buffer, Elution Buffer, Spin Columns, Collection Tubes.
Store at room temperature for up to 24 months.
Product Line: MethylCode™
Product Type: Kit, Bisulfite Conversion
Quantity: 50 Reactions
Sample Type: DNA (Genomic)
Shipping Condition: Room Temperature
Starting Material Cell No.: Purified genomic DNA - 200 ng to 500 ng
Format: Kit
Unit Size: Each
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Collaboration
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