Thermo Fisher, NP0007, NuPAGE™ LDS Sample Buffer (4X)

10mL NuPAGE LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels. It contains lithium dodecyl sulfate, pH 8. 4, which allows for maximum activity of the reducing agent. See all available buffers and reagents available for SDS-PAGENuPAGE LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels. To use:Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results. Note:NuPAGE LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. NuPAGE LDS Sample Buffer also contains a higher concentration glycerol. Buffer: Sample Loading Buffers
Concentration: 4 X
Gel Compatibility: NuPAGE™ Gels
Product Line: NuPAGE™
Product Type: LDS Sample Buffer
Quantity: 10mL
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Gel Type: NuPAGE
Unit Size: Each