Thermo Fisher, P36235, Premo Autophagy Assays with Sensor LC3B, p62, or Tandem Sensor RFP-GFP-LC3B

Green, LC3B-GFP, Premo Autophagy Sensor LC3B-GFP (BacMam 2.
0) Easily monitor various stages of cellular autophagy with Premo Autophagy assays and sensors, which are based on LC3B- or p62-linked fluorescent protein chimeras, or the RFP-GFP-LC3B tandem sensor. Premo Autophagy assay kits offer the selectivity of Premo Autophagy sensors, which are based on chimeras of autophagy-associated proteins LC3B or p62, and green (GFP) and/or red fluorescent protein (RFP), with the transduction efficiency of BacMam technology. Transduction or even co-transduction can be performed in a wide variety of cells, including neurons and neural stem cells (NSCs) using a simple protocol that involves adding the BacMam 2.
0 chimeric reagent to cells, incubating overnight, and then visualizing transduction using standard GFP (green fluorescent protein) and/or RFP (red fluorescent protein) settings.
Color: Green
Compatible Cells: Mammalian Cells
Description: Premo Autophagy Sensor LC3B-GFP (BacMam 2.
0)
Excitation/Emission: 488/520
For Use With (Equipment): Confocal Microscope
Kit Contents: LC3B-FP, a control BacMam LC3B (G120A)-RFP, and chloroquine diphosphate
Label Type: Other Label(s) or Dye(s)
Label or Dye: LC3B-GFP
Product Line: Premo
Product Type: Autophagy Sensor
Quantity: 1 kit
Shipping Condition: Wet Ice
Storage Requirements: Store at 2°C to 6°C, protected from light. Do Not Freeze.
Detection Method: Fluorescence
Format: Slide
Unit Size: Each