Thermo Fisher, PV3793, Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide

Z'-LYTE™ Kinase Assay Kit - Ser/Thr 13 Peptide A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon myosin light chain 2, containing Ser-20 and a Hyd-X-R-X-X-(S⁄T) consensus sequence, where Hyd is a hydrophobic residue and X is any residue.
Assay: Serine/Threonine Kinase Assay
Assay Entry: Biochemical phospho-specific activity (no antibody)
Excitation/Emission: 405⁄465, 535
For Use With (Application): FRET
Format: 384-well plate
Label or Dye: Methylcoumarin
No. of Reactions: 800 Reactions
Product Type: Kinase Assay Kit
Quantity: 800 x 20 μL Assays
Readout: End Point
References: JBC(2001) 276, p38956
Sample Type: myosin light chain 2 (KKRPQRRYSNVF)
Shipping Condition: Dry Ice
Substrate: Peptide-Based Substrate
Target: Serine/Threonine Kinases
Target Entry: ROCK2, MYLK2, DAPK3, CDC42BPA, CDC42BPB, CAMK4
Validated Kinases: CAMK4, CDC42BPA, CDC42BPB, DAPK3, MYLK2, ROCK2
Product Line: Z´-LYTE™
Unit Size: Each