Thermo Fisher, PV4407, Phosphate Sensor

100 nmol The Phosphate Sensor is a highly sensitive reagent for the detection of inorganic phosphate, either in real-time kinetic or end-point mode. This product is a purified form of recombinantE. coliphosphate-binding protein labeled with the fluorophore MDCC, which is sensitive to changes in its environment. Binding of inorganic phosphate by Phosphate Sensor is rapid and tight (Kd ∼ 0.
1 μM), resulting in a large increase in fluorescence. The Phosphate Sensor detects phosphate in the high-nanomolar to low-micromolar range, which is 100-fold more sensitive than malachite green. This reagent provides you with a sensitive assay for detecting enzymes that produce inorganic phosphate either directly or indirectly through coupled reactions, such as phosphatases, ATPases, GTPases, and phosphodiesterases.
Assay: Phosphatase Assay
Detection Method: Fluorescence
Excitation/Emission: 430⁄450
For Use With (Application): Fluorescence Intensity
For Use With (Equipment): Microplate Reader
Format: 384-well plate
Label or Dye: MDCC
Product Type: Phosphate Sensor
Quantity: 100 nmol; 10,000 assay(s)
Readout: Kinetic
Shipping Condition: Dry Ice
Target: GTPases, ATPases, Phosphatases, Phosphodiesterases
Unit Size: Each