Thermo Fisher, PV4409, LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit, goat This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15122:The LanthaScreen™ TR-FRET Retinoic Acid Receptor (RAR) alpha Coactivator Assay Kit provides a sensitive and robust method for high-throughput screening of potential RAR alpha ligands as agonists of coactivator recruitment. The homogeneous mix-and-read assay format results in a ligand EC50 composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
How it worksThe LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit includes a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human RAR alpha ligand-binding domain (RAR alpha-LBD) that is tagged with glutathione-S-transferase (GST). To assay, RAR alpha-LBD is added to ligand test compounds, followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-anti-GST antibody. After room temperature incubation, the TR-FRET 520:495 emission ratio is calculated and used to determine the EC50 from a dose response curve of the compound.
Contents and Storage:The LanthaScreen™ TR-FRET RAR alpha Coactivator Assay Kit contains RAR alpha-LBD (GST) protein, fluorescein-labeled D22 coactivator peptide, Tb-anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Assay Entry: Biochemical coregulator interaction
Detection Method: Fluorescence
For Use With (Application): Co-Factor Interaction Assay, TR-FRET
For Use With (Equipment): Microplate Reader
Gene ID (Entrez): 5914
Ligand: RAR alpha
No. of Assays: 800 x 20 μL assays
Packaging: 384-well Plate
Product Type: TR-FRET RAR Alpha Coactivator Assay Kit
Quantity: 800 x 20 μL assays
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: RARA, RAR alpha, NR1B1
Conjugate: Tb (Terbium)
Product Line: LanthaScreen™
Unit Size: Each