Thermo Fisher, PV4655, LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit, goat

LanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kit, goat This kit contains Goat Tb-Anti-GST antibody; other kit components are the same as kit A15128:The LanthaScreen™ TR-FRET LXR (liver X receptor) alpha Coactivator Assay provides a sensitive and robust method for high-throughput screening of potential LXR alpha ligands as agonists of coactivator recruitment. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and an LXR alpha ligand-binding domain (LBD) that is tagged with glutathione-S-transferase (GST), in a homogeneous mix-and-read assay format.
Inthe LanthaScreen™ TR-FRET LXR alpha Coactivator Assay, LXR alpha-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50from a dose response curve of the compound. Based on the biology of the LXR alpha-coactivator peptide interaction, this ligand EC50is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and recruit coactivator peptide (Figure 1).
Contents and Storage:TheLanthaScreen™ TR-FRET LXR alpha Coactivator Assay Kitcontains LXR alpha-LBD (GST) protein, fluorescently labeled TRAP220/DRIP-2 coactivator peptide, Tb-labeled anti-GST antibody, and buffers. Store components as indicated in the assay protocol (-80°C, -20°C, or +4°C).
Assay Entry: Biochemical coregulator interaction
Detection Method: Fluorescence
For Use With (Application): Co-Factor Interaction Assay, TR-FRET
For Use With (Equipment): Microplate Reader
Gene ID (Entrez): 10062
Ligand: LXR alpha
Packaging: 384-well Plate
Product Type: TR-FRET LXR Alpha Coactivator Assay Kit
Quantity: 800 x 20 μL assays
Readout: End Point
Shipping Condition: Dry Ice
Target Entry: LXRA, LXR alpha, NR1H3
Conjugate: Tb (Terbium)
Product Line: LanthaScreen™
Unit Size: Each